Articles

The Effects of Vitamin C in Sepsis and ARDS

March 7, 2020


[ Electronic Sounds ]>>Please, Barry, why don’t you start by
telling us a little bit of the background and the scientific rationale
behind vitamin C and sepsis?>>Okay. That’s important. We have been studying vitamin C in
my laboratory for about twelve years. And before we ever took vitamin C into
a clinical setting with human beings, we had done a very significant amount
of work in animal model of sepsis, okay. And this was an animal model that we created
in the mouse where feces were injected into the animal’s abdomen
to create bacterial sepsis.>>Mm.>>All animals, after receiving feces-induced
peritonitis, develop very significant sepsis with multiple organ failure and with acute lung
injury which is what we were planning to study.>>So this is a [inaudible] model, right?>>No. This was different. And the way this model was produced
we would go to the mouse cage. We would get feces pellets. We would put them in with sterile saline
sonicate it up to a gemish, centrifuge that down so that there was just a clear, brown fluid. We would take the clear, brown fluid,
put that in the refrigerator overnight. The organisms that were in the feces
would replicate, replicate, replicate. Then we would pull that fluid out and just a
tenth of an ml of that fluid would be injected into the peritoneum of the experimental mouse.>>And that was enough to give them
horrendous sepsis and multiple organ failure?>>Yes. All mice, unless they got
treatment, would die within 36 hours.>>Wow. Okay.>>Okay. And we had done a significant
amount of work at a molecular level with vitamin C before we had
turned to the mouse model.>>Okay.>>And I’ll never forget this. It was about twelve years ago. We had two cages. We had a cage of mice that just
got the feces-induced peritonitis. Then we had another cage of mice with
feces-induced peritonitis but 40 minutes after they got the feces we injected 200
micrograms per gram of body weight of the mouse of vitamin C. Put the cages aside. That was about 4:00 in the afternoon. I always get to work around
5:45 or 6:00 in the morning. And so the next morning I get to work and I’m
rushing into the laboratory and there in front of me was a cage full of dead mice and cage
full of mice that were running around eating and drinking, the mice who had received
the vitamin C. And it was like electricity. We knew that something had
gone on with the mouse model that interrupted the course of sepsis.>>And so for, as a preview for tomorrow, because my understanding is the results are
still very much embargoed, the study is going to be published somewhere I
take it, probably somewhere big?>>Yes. The study will be
published tomorrow morning in JAMA.>>Okay. So the study is going to be in JAMA. And unfortunately we can’t
say anything about the results yet because they’re still under embargo. So all I can do is plead to anybody who
is watching, anybody who is listening, please tune in to the large screen
tomorrow where you’ll be able to see, where you’ll be able to see Dr.
Fowler present the results live. All I’ll ask is one final question.>>You can ask as many questions as you want to.>>Yes. Do you still give it?>>Absolutely.>>I’ll end this, I’ll end the statement
there and I’ll just [inaudible]. Thank you so much.>>Yes.

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